optiprep (Abbott Laboratories)
86
Structured Review
Abbott Laboratories
optiprep
Optiprep, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/optiprep/optiprep/pm41371534-63-5-7
Average 86 stars, based on 1 article reviews
Optiprep, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/optiprep/optiprep/pm41371534-63-5-7
Average 86 stars, based on 1 article reviews
optiprep - by Bioz Stars,
2026-09
86/100 stars
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Molecular Weight:Article Title: Development of an Effective Isolation Method for Plasma Extracellular Vesicles Excluding Lipoproteins by Precipitation With Polyethylene Glycol Article Snippet: .. For experiments, we used reagents and antibody as follows: sodium heparin; polyethylene glycol (PEG) (molecular weight: 6000); biotin‐conjugated rat monoclonal anti‐CD9 antibody (30B) (1.2 mg/mL); sodium dodecyl sulphate (SDS); bromophenol blue (Fujifilm Wako Chemicals, Osaka, Japan); NaCl; manganese chloride tetrahydrate; phosphotungstic acid; 4% paraformaldehyde; dithiothreitol (Nacalai Tesque, Kyoto, Japan); phosphate‐buffered saline (PBS) (Sigma–Aldrich, St. Louis, MO, USA); magnesium chloride hexahydrate; trichloroacetic acid; acetone; tris(hydroxymethyl)aminomethane (Tris); HCl; glycerol; NaOH (Kanto Chemical, Tokyo, Japan); polystyrene beads (CPC100) (IZON, Christchurch, New Zealand); EM stainer (Nisshin‐EM, Tokyo, Japan); bovine albumin solution (2 mg/mL); fetal bovine serum (FBS) (Thermo Fisher Scientific, Waltham, MA, USA); HEPES (Dojindo, Kumamoto, Japan) and Saline:Article Title: Development of an Effective Isolation Method for Plasma Extracellular Vesicles Excluding Lipoproteins by Precipitation With Polyethylene Glycol Article Snippet: .. For experiments, we used reagents and antibody as follows: sodium heparin; polyethylene glycol (PEG) (molecular weight: 6000); biotin‐conjugated rat monoclonal anti‐CD9 antibody (30B) (1.2 mg/mL); sodium dodecyl sulphate (SDS); bromophenol blue (Fujifilm Wako Chemicals, Osaka, Japan); NaCl; manganese chloride tetrahydrate; phosphotungstic acid; 4% paraformaldehyde; dithiothreitol (Nacalai Tesque, Kyoto, Japan); phosphate‐buffered saline (PBS) (Sigma–Aldrich, St. Louis, MO, USA); magnesium chloride hexahydrate; trichloroacetic acid; acetone; tris(hydroxymethyl)aminomethane (Tris); HCl; glycerol; NaOH (Kanto Chemical, Tokyo, Japan); polystyrene beads (CPC100) (IZON, Christchurch, New Zealand); EM stainer (Nisshin‐EM, Tokyo, Japan); bovine albumin solution (2 mg/mL); fetal bovine serum (FBS) (Thermo Fisher Scientific, Waltham, MA, USA); HEPES (Dojindo, Kumamoto, Japan) and Article Title: Inflammation-associated molecules in the glomerular-endothelium in mild IgA-nephropathy patients identified by single-cell and spatial transcriptome Article Snippet: Fresh renal tissue was sectioned using a vibrating-blade microtome (Leica Microsystems, Wetzlar, Germany), dissociated in Dulbecco’s modified Eagle medium (Nacalai Tesque, Inc., Kyoto, Japan) containing 100 U/mL Collagenase D (F. Hoffmann-La Roche Ltd; [1108886600]) and 5 U of DNaseI (Takara Bio Inc., Shiga, Japan) by shaking at 37 °C for 60 min, and then filtered through a 40 μm cell strainer (Corning Inc., New York, U.S.). .. The resultant cell suspension was subjected to density-gradient centrifugation using Article Title: Inflammation-associated molecules in the glomerular-endothelium in mild IgA-nephropathy patients identified by single-cell and spatial transcriptome. Article Snippet: Fresh renal tissue was sectioned Communications Biology | (2025) 8:915 9 using a vibrating-blade microtome (Leica Microsystems, Wetzlar, Germany), dissociated in Dulbecco’s modified Eagle medium (Nacalai Tesque, Inc., Kyoto, Japan) containing 100U/mL Collagenase D (F. Hoffmann-La RocheLtd; [1108886600]) and5UofDNaseI (TakaraBio Inc., Shiga, Japan) by shaking at 37 °C for 60min, and then filtered through a 40 μm cell strainer (Corning Inc., New York, U.S.). .. The resultant cell suspension was subjected to density-gradient centrifugation using Suspension:Article Title: Inflammation-associated molecules in the glomerular-endothelium in mild IgA-nephropathy patients identified by single-cell and spatial transcriptome Article Snippet: Fresh renal tissue was sectioned using a vibrating-blade microtome (Leica Microsystems, Wetzlar, Germany), dissociated in Dulbecco’s modified Eagle medium (Nacalai Tesque, Inc., Kyoto, Japan) containing 100 U/mL Collagenase D (F. Hoffmann-La Roche Ltd; [1108886600]) and 5 U of DNaseI (Takara Bio Inc., Shiga, Japan) by shaking at 37 °C for 60 min, and then filtered through a 40 μm cell strainer (Corning Inc., New York, U.S.). .. The resultant cell suspension was subjected to density-gradient centrifugation using Article Title: Inflammation-associated molecules in the glomerular-endothelium in mild IgA-nephropathy patients identified by single-cell and spatial transcriptome. Article Snippet: Fresh renal tissue was sectioned Communications Biology | (2025) 8:915 9 using a vibrating-blade microtome (Leica Microsystems, Wetzlar, Germany), dissociated in Dulbecco’s modified Eagle medium (Nacalai Tesque, Inc., Kyoto, Japan) containing 100U/mL Collagenase D (F. Hoffmann-La RocheLtd; [1108886600]) and5UofDNaseI (TakaraBio Inc., Shiga, Japan) by shaking at 37 °C for 60min, and then filtered through a 40 μm cell strainer (Corning Inc., New York, U.S.). .. The resultant cell suspension was subjected to density-gradient centrifugation using Centrifugation:Article Title: Inflammation-associated molecules in the glomerular-endothelium in mild IgA-nephropathy patients identified by single-cell and spatial transcriptome Article Snippet: Fresh renal tissue was sectioned using a vibrating-blade microtome (Leica Microsystems, Wetzlar, Germany), dissociated in Dulbecco’s modified Eagle medium (Nacalai Tesque, Inc., Kyoto, Japan) containing 100 U/mL Collagenase D (F. Hoffmann-La Roche Ltd; [1108886600]) and 5 U of DNaseI (Takara Bio Inc., Shiga, Japan) by shaking at 37 °C for 60 min, and then filtered through a 40 μm cell strainer (Corning Inc., New York, U.S.). .. The resultant cell suspension was subjected to density-gradient centrifugation using Article Title: Legionella uses host Rab GTPases and BAP31 to create a unique ER niche. Article Snippet: .. The homogenate was centrifuged at 1,000 x g for 10 min, and the resulting post-nuclear supernatant (PNS) was layered onto a 0–28% Article Title: Legionella uses host Rab GTPases and BAP31 to create a unique ER niche Article Snippet: .. The homogenate was centrifuged at 1,000 × g for 10 min, and the resulting post-nuclear supernatant (PNS) was layered onto a 0–28% Article Title: Inflammation-associated molecules in the glomerular-endothelium in mild IgA-nephropathy patients identified by single-cell and spatial transcriptome. Article Snippet: Fresh renal tissue was sectioned Communications Biology | (2025) 8:915 9 using a vibrating-blade microtome (Leica Microsystems, Wetzlar, Germany), dissociated in Dulbecco’s modified Eagle medium (Nacalai Tesque, Inc., Kyoto, Japan) containing 100U/mL Collagenase D (F. Hoffmann-La RocheLtd; [1108886600]) and5UofDNaseI (TakaraBio Inc., Shiga, Japan) by shaking at 37 °C for 60min, and then filtered through a 40 μm cell strainer (Corning Inc., New York, U.S.). .. The resultant cell suspension was subjected to density-gradient centrifugation using Isolation:Article Title: Inhibition of NOD-like receptor pyrin domain-containing protein 3 inflammasome activation by MK-571, a multidrug resistance protein inhibitor. Article Snippet: Proinflammatory cytokines such as interleukin (IL)-1β and IL-18 are known to be activated through the NOD-like receptor pyrin domain-containing protein 3 (NLRP3) inflammasome, a multi-protein intracellular complex.. MK571 has been identified as an efflux inhibitor of multidrug resistance protein (MRP) transporters.. In this study, we have revealed that MK-571 effectively suppresses the activation of the NLRP3 inflammasome in bone marrow-derived macrophages (BMDMs) by elevating intracellular cAMP levels. Gradient Centrifugation:Article Title: Inhibition of NOD-like receptor pyrin domain-containing protein 3 inflammasome activation by MK-571, a multidrug resistance protein inhibitor. Article Snippet: Proinflammatory cytokines such as interleukin (IL)-1β and IL-18 are known to be activated through the NOD-like receptor pyrin domain-containing protein 3 (NLRP3) inflammasome, a multi-protein intracellular complex.. MK571 has been identified as an efflux inhibitor of multidrug resistance protein (MRP) transporters.. In this study, we have revealed that MK-571 effectively suppresses the activation of the NLRP3 inflammasome in bone marrow-derived macrophages (BMDMs) by elevating intracellular cAMP levels. other:Article Title: Pharmacological characterization of cannabidiol as a negative allosteric modulator of the 5-HT 2A receptor. Article Snippet: Promising clinical evidence suggests that psychedelic compounds, like lysergic acid diethylamide (LSD), have therapeutic value for treatment of psychiatric disorders.. However, they often produce hallucinations and dissociative states, likely mediated by the serotonin (5-HT) receptor 5-HT2A, raising challenges regarding therapeutic scalability.. Given the reported antipsychotic effects of cannabidiol (CBD) and its promiscuous binding at many receptors, we assessed whether CBD could modulate 5-HT2A signalling. |